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Antibodies bound to the blocking peptide now not bind on the epitope on the goal protein. By evaluating the staining with the blocked antibody as opposed to the antibody alone, one can see which staining is particular.
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This information outlines even further probable optimization of mobile and immunoassay parameters to ensure the best possible benefits are...
This tutorial outlines even more possible optimization of cellular and immunoassay parameters to guarantee the best possible final results are...
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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...
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Blocking peptides are peptides that bind particularly for the goal antibody and block antibody binding. These peptide generally consists of the epitope recognized by the antibody.
Target Information and facts Cyclin E belongs into the remarkably conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and get more info degradation patterns which contribute towards the temporal coordination of each and every mitotic occasion. Cyclin E forms a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress through S period.
Overexpression of this gene has long been observed in several tumors, which leads to chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was found to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a important function in endorsing cell-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are actually explained. Two more splice variants have been claimed but in depth nucleotide sequence info is not yet obtainable.
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